Coding

Part:BBa_K4449002:Design

Designed by: SRIJANI SAHA   Group: iGEM22_IISER_Berhampur   (2022-09-28)


Bhp


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1254
    Illegal EcoRI site found at 1982
    Illegal XbaI site found at 1792
    Illegal SpeI site found at 229
    Illegal SpeI site found at 607
    Illegal SpeI site found at 1750
    Illegal PstI site found at 380
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1254
    Illegal EcoRI site found at 1982
    Illegal SpeI site found at 229
    Illegal SpeI site found at 607
    Illegal SpeI site found at 1750
    Illegal PstI site found at 380
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1254
    Illegal EcoRI site found at 1982
    Illegal BamHI site found at 1611
    Illegal XhoI site found at 6904
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1254
    Illegal EcoRI site found at 1982
    Illegal XbaI site found at 1792
    Illegal SpeI site found at 229
    Illegal SpeI site found at 607
    Illegal SpeI site found at 1750
    Illegal PstI site found at 380
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1254
    Illegal EcoRI site found at 1982
    Illegal XbaI site found at 1792
    Illegal SpeI site found at 229
    Illegal SpeI site found at 607
    Illegal SpeI site found at 1750
    Illegal PstI site found at 380
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization was done by inserting two G bases at the 10 th and 11 th position of the designed primer sequence so as to maintain the reading frame of the sequence during cloning in expression vector Pet28b+.

Source

The coding sequence of this part has been retrieved in FASTA format from the European Nucleotide Archive browser.The accession number is AAK29746.1

References


1.Amirmorteza ,EbrahimzadehNamvar,Sara Bastarahang,Niloufar Abbasi,Ghazaleh Sheikhi,Ghehi ,Sara,Farhadbakhtiarian,Parastoo Arezi,Mahsa Hosseini,Sholeh Zaeemi Baravati, Zahra Jokar,Sara Ganji Chermahin ,Clinical characteristics of Staphylococcus epidermidis: a systematic review ,2014 Sep 30, GMS Hygiene and Infection Control 2014, Vol. 9(3), DOI: 10.3205/dgkh000243


2.Shankar Upadhyayula, Mamatha Kambalapalli, and Basim . Asmar, Staphylococcus epidermidis Urinary Tract Infection in an Infants;24 July 2012,Case Reports in Infectious Disease,Volume 2012, Article ID 983153. DOI: https://doi.org/10.1155/2012/983153
3.https://www.ebi.ac.uk/ena/browser/view/AAK29746.1